Nutrient and hormonal regulation of pyruvate kinase gene expression
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چکیده
منابع مشابه
Nutrient and hormonal regulation of pyruvate kinase gene expression.
Mammalian pyruvate kinase (PK), a key glycolytic enzyme, has two genes named PKL and PKM, which produce the L- and R-type isoenzymes by means of alternative promoters, and the M1-and M2-types by mutually exclusive alternative splicing respectively. The expression of these genes is tissue-specific and under developmental, dietary and hormonal control. The L-type isoenzyme (L-PK) gene contains mu...
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t w t r i p s a t p s b f c ransferase, EC 2.7.1.40), a rate-controlling glycolytic nzyme, catalyses the transfer of a phosphoryl group rom phosphoenolpyruvate to ADP, yielding ATP and yruvate. PK exists in the cytosol and is functional as tetramer. Mammalian PK exists as four isoenzymes, hich are referred to as the L-, R-, M1-, and M2-types, espectively (1). The L-, R-, and M1-PK isoenzymes ar...
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The majority of bacterial promoters are regulated by at least two transcription factors. Many of these promoters are regulated by two activators, with expression being co-dependent on the activity of both activators [l] . At most of these promoters, one of the activators responds to a ‘global’ regulatory signal whilst the other responds to a ‘specific’ signal. For example, transcription initiat...
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We show that the M2 isoform of pyruvate kinase (M2PYK) exists in equilibrium between monomers and tetramers regulated by allosteric binding of naturally occurring small-molecule metabolites. Phenylalanine stabilizes an inactive T-state tetrameric conformer and inhibits M2PYK with an IC50 value of 0.24 mM, whereas thyroid hormone (triiodo-L-thyronine, T3) stabilizes an inactive monomeric form of...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1999
ISSN: 0264-6021
DOI: 10.1042/0264-6021:3370001